Genetic mutations in HSV-1 replication-defective vectors: Implications for their safety in gene therapy applications

The results from this study demonstrate that monoclonal expansion of J∆NI8 HSV-1 vectors can result in genetic mutations, some of which can significantly alter the functional properties of the vector, in particular its fusogenic potential. These findings align with the known genomic instability of herpesviruses during replication, underscoring the importance of rigorous quality control in vector production to ensure therapeutic safety.

Some batches of HSV-1 replication-defective vectors may alter neuronal physiology

Whole-cell patch-clamp…