48
iPSC and their 3D HLC derivatives express markers of pluripotency and liver-like cells, respectively, and are highly permissive to LV transduction
Bulk cultures of male JHU106i iPSC were differentiated to 3D HLC spheroids as previously described expressing markers of pluripotency and hepatocytes, respectively [29, 30]. Cultures were infected with 2nd generation HIV-1 based LV vectors carrying native LTR (pHV) or SIN LTR (pHR) configurations, respectively, and use an SFFV internal promoter to drive GFP expression (Fig. S1A, B). Flow…