In vitro and in vivo rescue of dopaminergic neurons in Parkinson’s disease models after Parkin gene therapy

Preparation of PRKN expression plasmids

All expression plasmids were designed by the authors and were produced at GeneWiz (South Plainfield, NJ, USA). Expression cassettes consist of promoter, intron, human PRKN cDNA (NM_004562.3, transcript variant 1, PRKN-206) (1398 base pairs (bp)), and human growth hormone poly A (477 bp), which was inserted in pUC57. For promoters, chicken β-actin hybrid (CBh, 822 bp) [29], human Elongation Factor 1 alpha (EF1α, 1187 bp) [30], the short, intron-less variant of EF1α (EFS, 232 bp) [31], human…