SCOTCH – isoform-level characterization of gene expression through long-read single-cell RNA sequencing

Single-cell RNA sequencing helps researchers understand which genes are active in individual cells. However, many traditional methods rely on short sequencing reads, which can make it difficult to tell which full-length RNA isoforms are present. Isoforms are different RNA versions produced from the same gene, and they can have very different biological functions.

Researchers at the University of Pennsylvania and the Raymond G. Perelman Center for Cellular and Molecular Therapeutics at Children’s Hospital of Philadelphia, developed a new…