Unlocking data in Klebsiella lysogens to predict capsular type-specificity of phage depolymerases

A comprehensive profiling of prophage-encoded depolymerases targeting Klebsiella

To gather a collection of prophages with labeled KL type, all Klebsiella genomes were first downloaded from the NCBI database (n = 14,601), retaining those with a confident assigned KL type (n = 12,003). The screening of these bacterial genomes identified 77,802 prophages. Clusters of prophage sequence showing high identity (99% over more than 80% of their genome) were identified to remove redundancy, resulting in a total of…